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Proper Pipetting Guidelines

- We are ISO17025 Accredited -
Consistency in all aspects of pipetting procedure will significantly contribute to optimum reproducibility. Be sure to use:
  • Consistent pickup/dispense rhythm while pipetting.
  • Consistent speed and smoothness when you press and release pushbutton.
  • Consistent pushbutton pressure at the first stop.
  • Consistent immersion depth.
  • Minimal angle (less than 20 degrees from vertical).
Prevent liquids from being drawn into the pipette shaft by taking the following precautions:
  • Never invert or lay pipette down if liquid is in the tip.
  • For P-5000 and P-10ML model pipettes, use the special safety filters supplied.
  • Consistent pushbutton pressure at the first stop.
Use proper tips:
  • Tips must seal properly on the shaft to assure an air-tight seal and avoid leaks or poor accuracy.
  • Tips must be soft and flexible so that the shaft is not scratched or worn prematurely.